Using disposable impedance immunosensors, it is possible to measure and
quantify the bone formation biomarker alkaline phosphatase (ALP).
Platform for high resolution biochemical
imaging of surfaces and interfaces
Nanowell arrays can also be used as platforms for biological replication. Through nanowell arrays endowed with artificial cell membrane functions, it is possible to observe drug delivery and diagnostic processes in vitro. For this purpose, it is important to first understand the fluidity of proteins that become denatured by direct contact on the surface when forming the nanowell arrays that act as lipid membranes.
A two-step process has been introduced to manufacture artificial lipid raft bilayers. First, a self-assembly process is used to place a single layer of PEG lipopolymers and hydrophilic molecules inside the nanoparticles. Second, multiple liposomes are added to the wells. Then, through the vesicle fusion process, the liposomes rupture to form lipid raft bilayers. The image on the left shows the AFM image after the formation of the artificial lipid bilayer. Using the artificial lipid bilayer, the interaction between proteins through self-assembled protein nano arrays was studied step-by-step according to the size of the single protein array.
